Uso alternativo del colorante gelred en la tinción de ácidos nucleicos./alternative use of gelred dye in the staining nucleic acids

Nestor Javier Orozco Clavijo; Jhoan Camilo Herrera Cañón; Jhon Fredy Betancur Pérez

doi:10.30554/archmed.13.2.15.2013

Alternative use of Gelred dye in the staining nucleic acids

Uso alternativo del colorante gelred en la tinción de ácidos nucleicos./alternative use of gelred dye in the staining nucleic acids

Published 2013-12-15

https://doi.org/10.30554/archmed.13.2.15.2013

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Vol. 13 No. 2

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Artículos de Investigación

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Orozco Clavijo, N. J., Herrera Cañón, J. C., & Betancur Pérez, J. F. (2013). Alternative use of Gelred dye in the staining nucleic acids. Archivos De Medicina , 13(2), 160-166. https://doi.org/10.30554/archmed.13.2.15.2013

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https://doi.org/10.30554/archmed.13.2.15.2013

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How to Cite

Orozco Clavijo, N. J., Herrera Cañón, J. C., & Betancur Pérez, J. F. (2013). Alternative use of Gelred dye in the staining nucleic acids. Archivos De Medicina , 13(2), 160-166. https://doi.org/10.30554/archmed.13.2.15.2013

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Nestor Javier Orozco Clavijo

Estudiante de quinto semestres de medicina universidad de manizales.

Jhoan Camilo Herrera Cañón

Estudiante de quinto semestres de medicina universidad de manizales.

Jhon Fredy Betancur Pérez

Docente investigador universidad de manizales.

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Nestor Javier Orozco Clavijo,

Estudiante de quinto semestres de Medicina Universidad de Manizales.

Jhoan Camilo Herrera Cañón,

Estudiante de quinto semestres de Medicina Universidad de Manizales.

Jhon Fredy Betancur Pérez,

Biólogo, Especialista en biología molecular y biotecnología, doctor en ciencias

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Electrophoresis agarose gel

bromide ethidium

dyes.

Determine the reproducibility of DNA staining in agarose gels using the dye GelRedTM in order to replace ethidium bromide in the use of nucleic acids staining. Materials and Methods: The extraction of the total DNA from nine samples and the amplified DNA fragment of 494 pb of exon 15 associated with the APC gene from sixfragments between 150 pb – 350 pb associated with the hMLH-1 and MSH-2 genes was performed. Both preparations, total DNA and amplified DNA were visualized using GelRed and Ethidium bromide dye. Results: Both systems of coloration gave very similar results; the DNA can be visualized correctly without any difficulty. Conclusion: The GelRed dye is highly reproducible and its use is recommended including in DNA samples, thus reducing the contamination with dyes such as ethidium bromide.

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