Development and validation of an analytical method for the determination of ciprofloxacin in serum by High-Performance Liquid Chromatography with ultraviolet detection

Desarrollo y validación de un método analítico para la determinación de ciprofloxacina en suero por cromatografía liquida de alta precisión con detección ultravioleta./ development and validation of an analytical method for the determination of ciproflox

To develop a rapid and simple method for Ciprofloxacin determination in samples of whey to be used in pharmacokinetic studies. Ciprofloxacin is an antibiotic that belongs to the group of the fluoroquinolone, commonly used in Colombia with an
extensive antibacterial spectrum. Several High-Performance Liquid Chromatography (HPLC) methods have been reported for the analysis of ciprofloxacin in biological fluids. Materials and methods: the method used was High-Performance Liquid
Chromatography (HPLC) with ultraviolet detection to 280 nm, mobile phase isocratic, Dexamethasone as internal standard and a range of quantification from 0,1 to 10 μg/ ml. Results: The times of retention were 4,93 min and 15,71 min for Ciprofloxacin and Dexamethasone respectively, precision and accuracy were More than 95 % and a and elution time per sample was 20 minutes. Conclusions: Was developed and validated
a sensitive and accurate analytical methods for Ciprofloxacin determination in serum, It is the sufficiently reproducible to be used in pharmacokinetic studies