Análisis de las variantes genéticas IL-6 – 174G/C y FNT-α -308 G/A en pacientes con diagnóstico de sepsis bacteriana

Análisis de las variantes genéticas IL-6 – 174G/C y FNT-α -308 G/A en pacientes con diagnóstico de sepsis bacteriana

The objective of this study was to evaluate the IL-6 -174G/C and TNF-α -308G/A variants in patients diagnosed with bacterial sepsis in an Intensive Care Unit (ICU).

Materials and methods. A prospective observational study was conducted that included one hundred patients with sepsis and thirty control subjects. Blood samples were collected to measure the concentrations of cytokines IL-6 and TNF-α and to detect the genetic variants IL-6-174G/C and TNF-α-308A/G. A multivariate analysis was performed with cytokine levels, sepsis progression, comorbidities and mortality as dependent variables and genetic variants analyzed as independent variables. Survival analysis was performed according to the Kaplan and Meyer model.

Results. Multivariate analysis showed that the G allele of IL-6-174 G/C was a risk factor for developing sepsis due to infection by Gram-negative bacteria (62,3%; OR:2,619; IC95%1,095-6,259; p=0,030) and the GA genotype of TNF-α-308G/A was significantly associated with the development of septic shock (50%; OR:4,053; IC95%1,546- 10,623; p=0,004), arterial hypertension (53%; OR:3,359; IC 95% 1,253-9,008; p=0,016) and infection by Gram-positive bacteria (17,6%; OR:9,525; IC95%1,854-48,928; p=0,007). Conclusion. The GA genotype of TNF-α-308G/A was found to be a risk factor for the development of septic shock