Características moleculares y susceptibilidad a antibióticos de cepas de Staphylococcus aureus colonizantes de pacientes con patologías nasales procedentes de Cartagena (Colombia), 2015: estudio observacional

molecular characteristics and antibiotic susceptibility of Staphylococcus aureus strains colonizing patients with nasal pathologies

Publicado 2015-12-31


https://doi.org/10.30554/archmed.15.2.743.2015
Abrir | Descargar
PDF FLIP HTML
Número
Vol. 15 Núm. 2
Sección
Artículos de Investigación
Cómo citar
Montes, O., Milanés, R., Alvarez, A., Coronado, S., & Reyes, N. (2015). Características moleculares y susceptibilidad a antibióticos de cepas de Staphylococcus aureus colonizantes de pacientes con patologías nasales procedentes de Cartagena (Colombia), 2015: estudio observacional. Archivos De Medicina (Manizales), 15(2), 226-240. https://doi.org/10.30554/archmed.15.2.743.2015
Descargar cita
Endnote/Zotero/Mendeley (RIS) BibTeX
DOI

https://doi.org/10.30554/archmed.15.2.743.2015
Dimensions
PlumX

Cómo citar

Montes, O., Milanés, R., Alvarez, A., Coronado, S., & Reyes, N. (2015). Características moleculares y susceptibilidad a antibióticos de cepas de Staphylococcus aureus colonizantes de pacientes con patologías nasales procedentes de Cartagena (Colombia), 2015: estudio observacional. Archivos De Medicina (Manizales), 15(2), 226-240. https://doi.org/10.30554/archmed.15.2.743.2015

Descargar cita

Licencia
Creative Commons License

Esta obra está bajo una licencia internacional Creative Commons Atribución-NoComercial-SinDerivadas 4.0.

Oscar Montes
Rosa Milanés
Alvaro Alvarez
Sandra Coronado
Niradiz Reyes
Mostrar biografía de los autores

Artículos más leídos del mismo autor/a

Introducción

Staphylococcus aureus es uno de los principales patógenos tanto a nivel hospitalario como comunitario. La diseminación del clon ST8-IVc-PVL+ de Staphylococcus aureus meticilino resistente (SARM) genéticamente relacionado con el clon USA300 ha sido descrita en Colombia y otros países suramericanos.

 Objetivo: Determinar algunas características moleculares y los perfiles de resistencia a antibióticos de aislamientos de Staphylococcus aureus colonizantes de pacientes con patología nasal atendidos en el servicio de Otorrinolaringología del Hospital Universitario del Caribe de la ciudad de Cartagena, Colombia.

 Métodos: Los aislamientos de Staphylococcus aureus obtenidos de pacientes colonizados fueron sometidos a extracción de ADN y ensayos de PCR para determinar la presencia de los genes mecA y lukF-PV. Se determinó la pertenencia de las cepas a los complejos clonales CC5 y CC8, y se determinaron las relaciones clonales de los aislamientos mediante análisis de PFGE. A las cepas SARM se les tipificó y sub-tipificó el casete cromosomal estafilocócico SCCmec mediante PCR múltiple.

 Resultados: La prevalencia de colonización encontrada para Staphylococcus aureus fue 22.8% y 5.26% para SARM. Del total de aislamientos SARM, 33.3% estuvieron relacionados con el clon CC8-SARM-IVc y 22.28% con CC8-SARM-IVa. Los genes para la leucocidina PVL se identificaron en 66.7% de aislamientos SARM y 40% de aislamientos sensibles a meticilina (SASM). En cuanto a la distribución de los aislamientos SARM en complejos clonales, 41% pertenecieron al CC8 y 18% al CC5.

 

Conclusiones: Los clones CC8-SARM-IVc y CC8-SARM-IVa, ambos relacionados con el clon USA300, circulan en población con patología nasal de la ciudad de Cartagena de Indias. 

 

Palabras claves: USA300, portación nasal, SARM, SCCmec, PFGE.

Fuente: DeCS Server, BIREME

Visitas del artículo | Visitas PDF

Descargas

Los datos de descarga todavía no están disponibles.
  1. Deurenberg RH, Stobberingh EE. The evolution of Staphylococcus aureus. Infection, genetics and evolution. 2008;8(6):747-63.
  2. DeLeo FR, Otto M, Kreiswirth BN, Chambers HF. Community-associated meticillin-resistant Staphylococcus aureus. The Lancet. 2010;375(9725):1557-68.
  3. Guzmán-Blanco M, Mejía C, Isturiz R, Alvarez C, Bavestrello L, Gotuzzo E, et al. Epidemiology of meticillin-resistant Staphylococcus aureus (MRSA) in Latin America. International journal of antimicrobial agents. 2009;34(4):304-8.
  4. Pérez A, Dennis RJ, Rodrı́guez B, Castro AY, Delgado V, Lozano JM, et al. An interrupted time series analysis of parenteral antibiotic use in Colombia. Journal of clinical epidemiology. 2003;56(10):1013-20.
  5. Galvis C, Mariño A, Monroy J, Posso H. The impact of an antibiotic restriction policy in the intensive care unit at the hospital militar central. Revista Med. 2008;16(1):19-24.
  6. Saavedra C, Eslava J, Cuervo S, Quemba J, Cardona G. Correlación entre la disminución en el uso de cefalosporinas de tercera generación y la disminución de la resistencia de Klebsiella pneumoniae. Infectio. 2002;6(2):96.
  7. GIL D de M M. Staphylococcus aureus: Microbiología y aspectos moleculares de la resistencia a meticilina. Revista chilena de infectología. 2000;17:145-52.
  8. Walsh TR, Howe RA. The prevalence and mechanisms of vancomycin resistance in Staphylococcus aureus. Annual Reviews in Microbiology. 2002;56(1):657-75.
  9. Barber M. Methicillin-resistant staphylococci. Journal of Clinical Pathology. 1961;14(4):385.
  10. Rodríguez-Noriega E, Seas C. The changing pattern of methicillin-resistant Staphylococcus aureus clones in Latin America: implications for clinical practice in the region. Brazilian Journal of Infectious Diseases. 2010;14:87-96.
  11. Bettin A, Causil C, Reyes N. Molecular identification and antimicrobial susceptibility of Staphylococcus aureus nasal isolates from medical students in Cartagena, Colombia. Braz J Infect Dis. 2012 Jul-Aug;16(4):329-34.
  12. Millar BC, Jiru X, Moore JE, Earle JA. A simple and sensitive method to extract bacterial, yeast and fungal DNA from blood culture material. J Microbiol Methods. 2000 Oct;42(2):139-47.
  13. Zhang K, McClure JA, Elsayed S, Louie T, Conly JM. Novel multiplex PCR assay for characterization and concomitant subtyping of staphylococcal cassette chromosome mec types I to V in methicillin-resistant Staphylococcus aureus. J Clin Microbiol. 2005 Oct;43(10):5026-33.
  14. Brakstad OG, Aasbakk K, Maeland JA. Detection of Staphylococcus aureus by polymerase chain reaction amplification of the nuc gene. J Clin Microbiol. 1992 Jul;30(7):1654-60.
  15. Lina G, Piemont Y, Godail-Gamot F, Bes M, Peter MO, Gauduchon V, et al. Involvement of Panton-Valentine leukocidin-producing Staphylococcus aureus in primary skin infections and pneumonia. Clin Infect Dis. 1999 Nov;29(5):1128-32.
  16. Milheiriço C, Oliveira DC, de Lencastre H. Multiplex PCR strategy for subtyping the staphylococcal cassette chromosome mec type IV in methicillin-resistant Staphylococcus aureus:‘SCCmec IV multiplex’. Journal of antimicrobial chemotherapy. 2007;60(1):42-8.
  17. Escobar JA, Gómez IT, Murillo MJ, Castro BE, Chavarro B, Márquez RA, et al. Diseño de dos metodologías moleculares para la rápida identificación de aislamientos de Staphylococcus aureus resistente a meticilina asociados a la comunidad en Colombia. Biomédica. 2012;32(2):214-23.
  18. Maltezou HC, Giamarellou H. Community-acquired methicillin-resistant Staphylococcus aureus infections. International journal of antimicrobial agents. 2006;27(2):87-96.
  19. Otter J, French G. Community-associated meticillin-resistant Staphylococcus aureus strains as a cause of healthcare-associated infection. Journal of Hospital Infection. 2011;79(3):189-93.
  20. Dong L, Zhou X-C, Chen X-F, Yang J-H, Lin J, Zhang H-L, et al. [Detection of etiologic agents and antibiotic resistance in children with acute lower respiratory tract infection in Wenzhou City]. Zhongguo dang dai er ke za zhi= Chinese journal of contemporary pediatrics. 2006;8(5):369-72.
  21. Pinho MG, de Lencastre H, Tomasz A. An acquired and a native penicillin-binding protein cooperate in building the cell wall of drug-resistant staphylococci. Proceedings of the National Academy of Sciences. 2001;98(19):10886-91.
  22. Diekema D, Pfaller M, Schmitz F, Smayevsky J, Bell J, Jones R, et al. Survey of infections due to Staphylococcus species: frequency of occurrence and antimicrobial susceptibility of isolates collected in the United States, Canada, Latin America, Europe, and the Western Pacific region for the SENTRY Antimicrobial Surveillance Program, 1997–1999. Clinical Infectious Diseases. 2001;32(Supplement 2):S114-S32.
  23. Holmes RL, Jorgensen JH. Inhibitory activities of 11 antimicrobial agents and bactericidal activities of vancomycin and daptomycin against invasive methicillin-resistant Staphylococcus aureus isolates obtained from 1999 through 2006. Antimicrobial agents and chemotherapy. 2008;52(2):757-60.
  24. Huang H, Flynn NM, King JH, Monchaud C, Morita M, Cohen SH. Comparisons of community-associated methicillin-resistant Staphylococcus aureus (MRSA) and hospital-associated MRSA infections in Sacramento, California. Journal of clinical microbiology. 2006;44(7):2423-7.
  25. Freitas F, Guedes‐Stehling E, Siqueira‐Junior J. Resistance to gentamicin and related aminoglycosides in Staphylococcus aureus isolated in Brazil. Letters in applied microbiology. 1999;29(3):197-201.
  26. Onwubiko NE, Sadiq NM. Antibiotic sensitivity pattern of Staphylococcus aureus from clinical isolates in a tertiary health institution in Kano, Northwestern Nigeria. Pan African Medical Journal. 2011;8(1).
  27. Steinkraus G, White R, Friedrich L. Vancomycin MIC creep in non-vancomycin-intermediate Staphylococcus aureus (VISA), vancomycin-susceptible clinical methicillin-resistant S. aureus (MRSA) blood isolates from 2001–05. Journal of Antimicrobial Chemotherapy. 2007;60(4):788-94.
  28. Gobernado M. Resistencia en Staphylococcus aureus. Ahora a la vancomicina. Rev Esp Quimioter. 2002;15(3).
  29. Control CfD, Prevention. Vancomycin-resistant Staphylococcus aureus--Pennsylvania, 2002. MMWR Morbidity and mortality weekly report. 2002;51(40):902.
  30. Park K-H, Kim ES, Kim HS, Park S-J, Bang KM, Park HJ, et al. Comparison of the clinical features, bacterial genotypes and outcomes of patients with bacteraemia due to heteroresistant vancomycin-intermediate Staphylococcus aureus and vancomycin-susceptible S. aureus. Journal of antimicrobial chemotherapy. 2012;67(8):1843-9.
  31. Mamani E, Luján D, Pajuelo G, editors. Perfil de sensibilidad y resistencia de Staphylococcus aureus: Experiencia en el Hospital Nacional Hipólito Unanue. Anales de la Facultad de Medicina; 2006: UNMSM. Facultad de Medicina.
  32. Fiebelkorn K, Crawford S, McElmeel M, Jorgensen J. Practical disk diffusion method for detection of inducible clindamycin resistance in Staphylococcus aureus and coagulase-negative staphylococci. Journal of clinical microbiology. 2003;41(10):4740-4.
  33. Patel M, Waites KB, Moser SA, Cloud GA, Hoesley CJ. Prevalence of inducible clindamycin resistance among community-and hospital-associated Staphylococcus aureus isolates. Journal of clinical microbiology. 2006;44(7):2481-4.
  34. Chavez-Bueno S, Bozdogan B, Katz K, Bowlware KL, Cushion N, Cavuoti D, et al. Inducible clindamycin resistance and molecular epidemiologic trends of pediatric community-acquired methicillin-resistant Staphylococcus aureus in Dallas, Texas. Antimicrobial agents and chemotherapy. 2005;49(6):2283-8.
  35. Cheng H, Yuan W, Zeng F, Hu Q, Shang W, Tang D, et al. Molecular and phenotypic evidence for the spread of three major methicillin-resistant Staphylococcus aureus clones associated with two characteristic antimicrobial resistance profiles in China. Journal of Antimicrobial Chemotherapy. 2013;68(11):2453-7.
  36. Karlowsky JA, Adam HJ, Desjardins M, Lagacé-Wiens PR, Hoban DJ, Zhanel GG, et al. Changes in fluoroquinolone resistance over 5 years (CANWARD 2007–11) in bacterial pathogens isolated in Canadian hospitals. Journal of Antimicrobial Chemotherapy. 2013;68(suppl 1):i39-i46.
  37. Hussain FM, Boyle-Vavra S, Daum RS. Community-acquired methicillin-resistant Staphylococcus aureus colonization in healthy children attending an outpatient pediatric clinic. Pediatr Infect Dis J. 2001 Aug;20(8):763-7.
  38. Onanuga A, Temedie TC. Nasal carriage of multi-drug resistant Staphylococcus aureus in healthy inhabitants of Amassoma in Niger delta region of Nigeria. Afr Health Sci. 2011 Jun;11(2):176-81.
  39. Aubry-Damon H, Legrand P, Brun-Buisson C, Astier A, Soussy C-J, Leclercq R. Reemergence of gentamicin-susceptible strains of methicillin-resistant Staphylococcus aureus: roles of an infection control program and changes in aminoglycoside use. Clinical infectious diseases. 1997;25(3):647-53.
  40. Cauwelier B, Gordts B, Descheemaecker P, Van Landuyt H. Evaluation of a disk diffusion method with cefoxitin (30 μg) for detection of methicillin-resistant Staphylococcus aureus. European Journal of Clinical Microbiology and Infectious Diseases. 2004;23(5):389-92.
  41. Labandeira-Rey M, Couzon F, Boisset S, Brown EL, Bes M, Benito Y, et al. Staphylococcus aureus Panton-Valentine leukocidin causes necrotizing pneumonia. Science. 2007;315(5815):1130-3.
  42. Adler A, Temper V, Block CS, Abramson N, Moses AE. Panton-Valentine leukocidin-producing Staphylococcus aureus. Emerg Infect Dis. 2006;12:1789-90.
  43. Cupane L, Pugacova N, Berzina D, Cauce V, Gardovska D, Miklaševics E. Patients with Panton-Valentine leukocidin positive Staphylococcus aureus infections run an increased risk of longer hospitalisation. International journal of molecular epidemiology and genetics. 2012;3(1):48.
  44. Lina G, Piémont Y, Godail-Gamot F, Bes M, Peter M-O, Gauduchon V, et al. Involvement of Panton-Valentine leukocidin—producing Staphylococcus aureus in primary skin infections and pneumonia. Clinical Infectious Diseases. 1999;29(5):1128-32.
  45. Bocchini CE, Hulten KG, Mason EO, Gonzalez BE, Hammerman WA, Kaplan SL. Panton-Valentine leukocidin genes are associated with enhanced inflammatory response and local disease in acute hematogenous Staphylococcus aureus osteomyelitis in children. Pediatrics. 2006;117(2):433-40.
  46. Gillet Y, Vanhems P, Lina G, Bes M, Vandenesch F, Floret D, et al. Factors predicting mortality in necrotizing community-acquired pneumonia caused by Staphylococcus aureus containing Panton-Valentine leukocidin. Clinical Infectious Diseases. 2007;45(3):315-21.
  47. Zetola N, Francis JS, Nuermberger EL, Bishai WR. Community-acquired meticillin-resistant Staphylococcus aureus: an emerging threat. The Lancet infectious diseases. 2005;5(5):275-86.
  48. Jiménez JN, Ocampo AM, Vanegas JM, Rodríguez EA, Garcés CG, Patiño LA, et al. Characterisation of virulence genes in methicillin susceptible and resistant Staphylococcus aureus isolates from a paediatric population in a university hospital of Medellín, Colombia. Memórias do Instituto Oswaldo Cruz. 2011;106(8):980-5.
  49. Alvarez-Olmos MI, Enríquez SP, Pérez-Roth E, Méndez-Alvarez S, Escobar J, Vanegas N, et al. Pediatric cases from Colombia caused by a Panton-Valentine Leukocidin-positive community-acquired methicillin-resistant Staphylococcus aureus ST8-SCCmecIVc clone. The Pediatric infectious disease journal. 2009;28(10):935.
  50. Qi W, Ender M, O'Brien F, Imhof A, Ruef C, McCallum N, et al. Molecular epidemiology of methicillin-resistant Staphylococcus aureus in Zürich, Switzerland (2003): prevalence of type IV SCCmec and a new SCCmec element associated with isolates from intravenous drug users. Journal of clinical microbiology. 2005;43(10):5164-70.
  51. Cruz C, Moreno J, Renzoni A, Hidalgo M, Reyes J, Schrenzel J, et al. Tracking methicillin-resistant Staphylococcus aureus clones in Colombian hospitals over 7 years (1996–2003): emergence of a new dominant clone. International journal of antimicrobial agents. 2005;26(6):457-62.
  52. Alvarez CA, Barrientes OJ, Leal AL, Contreras GA, Barrero L, Rincón S, et al. Community-associated methicillin-resistant Staphylococcus aureus, Colombia. Emerging infectious diseases. 2006;12(12):2000.
  53. Reyes J, Rincón S, Díaz L, Panesso D, Contreras GA, Zurita J, et al. Dissemination of methicillin-resistant Staphylococcus aureus USA300 sequence type 8 lineage in Latin America. Clinical infectious diseases. 2009;49(12):1861-77.
  54. Alvarez CA, Yomayusa N, Leal AL, Moreno J, Mendez-Alvarez S, Ibañez M, et al. Nosocomial infections caused by community-associated methicillin-resistant Staphylococcus aureus in Colombia. American journal of infection control. 2010;38(4):315-8.
  55. Popovich KJ, Weinstein RA, Hota B. Are community-associated methicillin-resistant Staphylococcus aureus (MRSA) strains replacing traditional nosocomial MRSA strains? Clinical Infectious Diseases. 2008;46(6):787-94.
  56. Donnio P-Y, Preney L, Gautier-Lerestif A-L, Avril J-L, Lafforgue N. Changes in staphylococcal cassette chromosome type and antibiotic resistance profile in methicillin-resistant Staphylococcus aureus isolates from a French hospital over an 11 year period. Journal of Antimicrobial Chemotherapy. 2004;53(5):808-13.
  57. Denis O, Deplano A, De Ryck R, Nonhoff C, Struelens MJ. Emergence and spread of gentamicin-susceptible strains of methicillin-resistant Staphylococcus aureus in Belgian hospitals. Microbial drug resistance. 2003;9(1):61-71.
  58. Nimmo G. USA300 abroad: global spread of a virulent strain of community‐associated methicillin‐resistant Staphylococcus aureus. Clinical Microbiology and Infection. 2012;18(8):725-34.
  59. Okuma K, Iwakawa K, Turnidge JD, Grubb WB, Bell JM, O'Brien FG, et al. Dissemination of new methicillin-resistant Staphylococcus aureus clones in the community. Journal of Clinical Microbiology. 2002;40(11):4289-94.
  60. MC D'Agata E, Webb GF, Horn MA, Moellering RC, Ruan S. Modeling the invasion of community-acquired methicillin-resistant Staphylococcus aureus into hospitals. Clinical Infectious Diseases. 2009;48(3):274-84.
  61. Huang Y, Tseng S, Hu J, Tsai J, Hsueh P, Teng L. Clonal spread of SCCmec type IV methicillin‐resistant Staphylococcus aureus between community and hospital. Clinical microbiology and infection. 2007;13(7):717-24.
  62. Gomes A, Santos Sanches I, Aires de Sousa M, Castaneda E, De Lencastre H. Molecular epidemiology of methicillin-resistant Staphylococcus aureus in Colombian hospitals: dominance of a single unique multidrug-resistant clone. Microbial Drug Resistance. 2001;7(1):23-32.
  63. Majeed Z, Arafat Y, Ajab Z, Akbar Malik S, Shehzad Abbasi W, Ajab H. Caracterización genotípica de Staphylococcus aureus resistente a la meticilina prevalente en hospitales de Pakistán. Acta bioquímica clínica latinoamericana. 2012;46(2):257-70.
  64. Becker A, Santos O, Castrucci F, Dias C, D'azevedo PA. First report of methicillin-resistant Staphylococcus aureus Cordobes/Chilean clone involved in nosocomial infections in Brazil. Epidemiology and infection. 2012;140(08):1372-5.
  65. Arias CA, Rincon S, Chowdhury S, Martínez E, Coronell W, Reyes J, et al. MRSA USA300 clone and VREF—a US–Colombian connection? New England Journal of Medicine. 2008;359(20):2177-9.
  66. Moran GJ, Krishnadasan A, Gorwitz RJ, Fosheim GE, McDougal LK, Carey RB, et al. Methicillin-resistant S. aureus infections among patients in the emergency department. New England Journal of Medicine. 2006;355(7):666-74.
  67. Chua K, Laurent F, Coombs G, Grayson ML, Howden BP. Not community-associated methicillin-resistant staphylococcus aureus (CA-MRSA)! A clinician's guide to community MRSA-Its evolving antimicrobial resistance and implications for therapy. Clinical Infectious Diseases. 2011;52(1):99-114.
  68. Rodríguez-Noriega E, Seas C, Guzmán-Blanco M, Mejía C, Alvarez C, Bavestrello L, et al. Evolution of methicillin-resistant Staphylococcus aureus clones in Latin America. International Journal of Infectious Diseases. 2010;14(7):e560-e6.
  69. Briles DE, Novak L, Hotomi M, van Ginkel FW, King J. Nasal colonization with Streptococcus pneumoniae includes subpopulations of surface and invasive pneumococci. Infection and immunity. 2005;73(10):6945-51.
  70. Rebollo-Pérez J, Ordoñez-Tapia C, Herazo-Herazo C, Reyes-Ramos N. Nasal carriage of Panton Valentine leukocidin-positive methicillin-resistant Staphylococcus aureus in healthy preschool children. Revista de Salud Pública. 2011;13(5):824-32.

Información

Perfil de Google Académico

Guía de autores