molecular characteristics and antibiotic susceptibility of Staphylococcus aureus strains colonizing patients with nasal pathologies

Características moleculares y susceptibilidad a antibióticos de cepas de Staphylococcus aureus colonizantes de pacientes con patologías nasales procedentes de Cartagena (Colombia), 2015: estudio observacional

Staphylococcus aureus is a major pathogen both hospital and community level. The spread of clone ST8-IVc-PVL + genetically Staphylococcus aureus methicillin resistant (MRSA) related to the USA300 clone has been reported in Colombia and other South American countries

To determine some molecular characteristics and antibiotic resistance profiles of Staphylococcus aureus colonizing patients with nasal pathology attended at the University Hospital of Otolaryngology Caribbean city of Cartagena, Colombia.

Methods: Staphylococcus aureus isolates obtained from colonized patients were subjected to DNA extraction and PCR assays for the presence of the mecA gene and LukF-PV. Strains belonging to clonal complexes CC5 and CC8 was determined, and clonal isolates ratios were determined by PFGE analysis. A MRSA strains were typified and sub-typified the staphylococcal chromosomal cassette SCCmec by multiple PCR.

Results: The prevalence of Staphylococcus aureus colonization was found for 22.8% and 5.26% for MRSA. Of all MRSA isolates, 33.3% were related to CC8-MRSA clone-IVc and 22.28% with CC8-MRSA-IVa. The genes for PVL leukocidin identified in 66.7% of MRSA isolates and 40% of isolates sensitive aureus (MSSA). As for the distribution of MRSA isolates in complex clonal belonged to CC8 41% and 18% to CC

Conclusions: CC8-MRSA-IVc and IVa-CC8-MRSA clones, both related to the USA300 clone, circulating in people with nasal pathology of the city of Cartagena de Indias.